Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140440_1ekx CCATACCCCCAAGACTCCCCTTGGCCACAGTG(+AAAAAA)GTACCCTCCAGCTGCCCTTGGGCCCCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606140438_zssa GCCAGCTGCTGGGGCCCAAGGGCAGCTGGAGG(+AAAAAA)GTACCACTGTGGCCAAGGGGAGTCTTGGGGG NGG 10 8 17 8 24 0 100 5 10
    240606140437_zda7 TAACAGTGAACACAGCAAGGTCTGTGTTATGG(+AAAAAA)GATCCTTTTCCCAAAACAAACAAACAAACAA NGG 10 8 17 8 24 0 100 5 10
    240606140435_8qe9 TTTGTTTGTTTGTTTGTTTGTTTTGGGAAAAG(+AAAAAA)GATCCCATAACACAGACCTTGCTGTGTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606140434_lv5q CTGGGGAGACTGTAGGAATGATATTTCTCAAG(+AAAAAA)GTACCTCTGAAGCTGTAAGAACACTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606140433_r923 TCCCAGAGCCCAGTGTTCTTACAGCTTCAGAG(+AAAAAA)GTACCTTGAGAAATATCATTCCTACAGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606140432_82y1 ATCAATGAGAGTCATTGATCTCGCACCATGTG(+AAAAAA)GATCCTGGGGATTGAATTTGGATTTATTAGA NGG 10 8 17 8 24 0 100 5 10
    240606140429_23nl CCAAGTCTAATAAATCCAAATTCAATCCCCAG(+AAAAAA)GATCCACATGGTGCGAGATCAATGACTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606140427_791m TCTCTCCAGTCTCCATTATCTGTTCTTTTATG(+AAAAAA)GATCCCAAAAGATTAGTCATATACAAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606140426_egjt GGTAAAGCCTTTGTATATGACTAATCTTTTGG(+AAAAAA)GATCCATAAAAGAACAGATAATGGAGACTGG NGG 10 8 17 8 24 0 100 5 10