Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140455_46lo AGTCGCAGGAGGCAGGCGTGGCCCTTTCCCAG(+AAAAAA)GATCCTCACAGACAGATGTGGGAAGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606140454_45zt GATGGCACCTCCTTCCCACATCTGTCTGTGAG(+AAAAAA)GATCCTGGGAAAGGGCCACGCCTGCCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606140453_f3f9 AGGCTTCTATCTGCTCTCAGATGTCTGCTGAG(+AAAAAA)GATCCACCACTCACTGCTCTTGCTCTGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606140451_2bz3 CTCAGCACCCAGAGCAAGAGCAGTGAGTGGTG(+AAAAAA)GATCCTCAGCAGACATCTGAGAGCAGATAGA NGG 10 8 17 8 24 0 100 5 10
    240606140449_d1of CTGTCACCCAGCACTGGCAAGGAAGACAGGAG(+AAAAAA)GATCCATGAGGCTGACTGGCTAGCAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606140448_al91 AGCACTAGGCTTGCTAGCCAGTCAGCCTCATG(+AAAAAA)GATCCTCCTGTCTTCCTTGCCAGTGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606140446_dw1q AAAAGGAGGGTTGTTGGGGTGAGGAGAAAAGG(+AAAAAA)GTACCCCTCGAAGAAGCTCCTCAACCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606140445_rdcl ACCTTCCAGGGGTTGAGGAGCTTCTTCGAGGG(+AAAAAA)GTACCCTTTTCTCCTCACCCCAACAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240606140443_o1qa CACAGCAGCACAAACGGACTGTGGAGACACAG(+AAAAAA)GTACCAGTTCATGAATATTCTGGGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240606140442_l9c6 GAGAGATAGAAACCCAGAATATTCATGAACTG(+AAAAAA)GTACCTGTGTCTCCACAGTCCGTTTGTGCTG NGG 10 8 17 8 24 0 100 5 10