Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140051_3v0m GCTTGGCCGGATGATGTACCACTAAGACCGGG(+AAAAAA)GTACCTCTTGTGAAGACACTTTGTTGCTGTG NGG 10 8 17 8 24 0 100 5 10
    240606140050_ui1e CTGGTCACAGCAACAAAGTGTCTTCACAAGAG(+AAAAAA)GTACCCCGGTCTTAGTGGTACATCATCCGGC NGG 10 8 17 8 24 0 100 5 10
    240606140049_l3f2 GCTCAAGGCTGTGGAGCCACAGAACTGCTGAG(+AAAAAA)GTACCACAGGATGCCTCTGTGTAAGCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240606140047_49zy CAGGTCCACAGCTTACACAGAGGCATCCTGTG(+AAAAAA)GTACCTCAGCAGTTCTGTGGCTCCACAGCCT NGG 10 8 17 8 24 0 100 5 10
    240606140045_947z GGTCCCTAGTTTGCAAATTCAGTGTTTGGGTG(+AAAAAA)GATCCGTTCCTAGGGTACCTAAGATGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606140044_1gbt CTCCCTCAAACATCTTAGGTACCCTAGGAACG(+AAAAAA)GATCCACCCAAACACTGAATTTGCAAACTAG NGG 10 8 17 8 24 0 100 5 10
    240606140043_w2g2 CTGGAGATTGACCTGTGTAAAGTAACGTTTAG(+AAAAAA)GATCCTGACGTGACTGTCATCTCCAAGACTT NGG 10 8 17 8 24 0 100 5 10
    240606140042_zi2u GGAGGAAGTCTTGGAGATGACAGTCACGTCAG(+AAAAAA)GATCCTAAACGTTACTTTACACAGGTCAATC NGG 10 8 17 8 24 0 100 5 10
    240606140040_wqs8 GTGACTCTGGGGTTCAAGGCAGAGACTATGTG(+AAAAAA)GATCCCAGGGTCATACCGCCAAGCCAGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606140037_kg7z TCAGCTAGACTGGCTTGGCGGTATGACCCTGG(+AAAAAA)GATCCACATAGTCTCTGCCTTGAACCCCAGA NGG 10 8 17 8 24 0 100 5 10