Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140036_vkor TCAGCACCCAAGATTGGGGGATAACTAGACTG(+AAAAAA)GTACCAGTTCATATATGCCTTATAAAATCTG NGG 10 8 17 8 24 0 100 5 10
    240606140035_2sw1 TCAAACAGATTTTATAAGGCATATATGAACTG(+AAAAAA)GTACCAGTCTAGTTATCCCCCAATCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606140033_krvs TCTGTACCCACTGTCTGTCTGCTCCCCCGATG(+AAAAAA)GTACCTAAGGCAGCCTGCTGAATATACCCAC NGG 10 8 17 8 24 0 100 5 10
    240606140031_e8sf AGGTTGTGGGTATATTCAGCAGGCTGCCTTAG(+AAAAAA)GTACCATCGGGGGAGCAGACAGACAGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606140029_nfsy CCAGGAGGCCTGACCTGGCCGGAGTGGACAGG(+AAAAAA)GATCCAGAAACGAAGCCAAATAAGGGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606140028_b4mi GTGGCCTCTTCCCTTATTTGGCTTCGTTTCTG(+AAAAAA)GATCCCTGTCCACTCCGGCCAGGTCAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606140025_aau6 GTCTGCAACTATCCCTAACTCCAGGTCCAGGG(+AAAAAA)GATCCCATGCCGTCTTCTGACTTCTGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606140023_30t6 CTTGTGCCCTCAGAAGTCAGAAGACGGCATGG(+AAAAAA)GATCCCCTGGACCTGGAGTTAGGGATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606140022_1xbg GCACCAGCAGCATCCAATCAACCTGTACTATG(+AAAAAA)GATCCTCCAACATCTAAGCAATAATGCCCTA NGG 10 8 17 8 24 0 100 5 10
    240606140020_bxcz AGCTGTAGGGCATTATTGCTTAGATGTTGGAG(+AAAAAA)GATCCATAGTACAGGTTGATTGGATGCTGCT NGG 10 8 17 8 24 0 100 5 10