Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140019_00m6 ACACATTTGTGATAAGACAAGGAAGTCTACAG(+AAAAAA)GATCCTCCATCTCTTAGTCTTGCTGAGGTCA NGG 10 8 17 8 24 0 100 5 10
    240606140018_08en TGAAATGACCTCAGCAAGACTAAGAGATGGAG(+AAAAAA)GATCCTGTAGACTTCCTTGTCTTATCACAAA NGG 10 8 17 8 24 0 100 5 10
    240606140016_lzl3 GGAGGGGACCCGATTCCCGCCAAATAATCTCG(+AAAAAA)GATCCAGTAGCAGGGTGAGCACGTGATGGCT NGG 10 8 17 8 24 0 100 5 10
    240606140015_3joi TCTGAAGCCATCACGTGCTCACCCTGCTACTG(+AAAAAA)GATCCGAGATTATTTGGCGGGAATCGGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606140014_c36g AAAGCCTTGTGACATAGCTGTCCAAACATTTG(+AAAAAA)GATCCTTTAGCCACAGGATGACCCACTTGTC NGG 10 8 17 8 24 0 100 5 10
    240606140012_qrrt TCTTAGACAAGTGGGTCATCCTGTGGCTAAAG(+AAAAAA)GATCCAAATGTTTGGACAGCTATGTCACAAG NGG 10 8 17 8 24 0 100 5 10
    240606140011_fino TATTTCTCTCATTTGAAGATTTTCTCAACAGG(+AAAAAA)GTACCTCCTCTGAACAACAAGCTTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240606140009_c87m TGAGAATGTGGGAAGCTTGTTGTTCAGAGGAG(+AAAAAA)GTACCCTGTTGAGAAAATCTTCAAATGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606140008_uapn TGAAATGGCCTCAGCAAGAATAAAAGATGAAG(+AAAAAA)GATCCTGTGGACTTTCTTCTCCTGCTACAAA NGG 10 8 17 8 24 0 100 5 10
    240606140006_j8n8 ATACATTTGTAGCAGGAGAAGAAAGTCCACAG(+AAAAAA)GATCCTTCATCTTTTATTCTTGCTGAGGCCA NGG 10 8 17 8 24 0 100 5 10