Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135932_7chm AGCTTGCCCTCCTCCCTGCCAGGGCGGGGAAG(+AAAAAA)GATCCCTTCTGTTCCTTGTGGACTCCGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606135930_uax0 GCAGTAGCTCGGAGTCCACAAGGAACAGAAGG(+AAAAAA)GATCCTTCCCCGCCCTGGCAGGGAGGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606135929_lp3m GCTGAAGTTACAGCTCTACCTGCCTGATTTGG(+AAAAAA)GATCCAAACTCTGCAAGCAGGGAGTGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606135928_001s CAATTAAGAGCACTCCCTGCTTGCAGAGTTTG(+AAAAAA)GATCCCAAATCAGGCAGGTAGAGCTGTAACT NGG 10 8 17 8 24 0 100 5 10
    240606135926_9sbl ACCACATAGTGGCTCATAACCATCTGTAATGG(+AAAAAA)GATCCAATGCCATCTTCTGGTATCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240606135925_2hdh GCTGTCTTCAGAGATACCAGAAGATGGCATTG(+AAAAAA)GATCCCATTACAGATGGTTATGAGCCACTAT NGG 10 8 17 8 24 0 100 5 10
    240606135924_9lqg AGTCACCGCCTGGCAAGGGCCATGGTAGTTAG(+AAAAAA)GTACCGAAATGCTCCTGTACAGATGCAAACC NGG 10 8 17 8 24 0 100 5 10
    240606135922_sdli GTACAGGTTTGCATCTGTACAGGAGCATTTCG(+AAAAAA)GTACCTAACTACCATGGCCCTTGCCAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240606135921_2mgp CTAGCTGGGAGTGCCGGCAGAGGCAGGGCTGG(+AAAAAA)GTACCACAGGGCCAGCTTGAGTGTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606135919_iaz6 CAAGTTCCTCCCACACTCAAGCTGGCCCTGTG(+AAAAAA)GTACCCAGCCCTGCCTCTGCCGGCACTCCCA NGG 10 8 17 8 24 0 100 5 10