Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135827_ldkt GATCCCAGTTACTTGGGAGGCTGAGGGGTTAG(+AAAAAA)GATCCCAAATTCCAAGGCCTGTCTGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240606135825_i0sv TCACTTTATTCCAGACAGGCCTTGGAATTTGG(+AAAAAA)GATCCTAACCCCTCAGCCTCCCAAGTAACTG NGG 10 8 17 8 24 0 100 5 10
    240606135823_7orx TTCCCAGTAAAGCTCGGAGAGGCAGCTGCCAG(+AAAAAA)GATCCCACGGGAAGTATTTCCAAGTGTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606135821_r4fu TAGAAACCCACACTTGGAAATACTTCCCGTGG(+AAAAAA)GATCCTGGCAGCTGCCTCTCCGAGCTTTACT NGG 10 8 17 8 24 0 100 5 10
    240606135820_t4jj TGCCACCACCAGAGTCCATGCACCTCAACGTG(+AAAAAA)GATCCACTTGGGTCCTGGCATCTTGAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606135818_oink ATAAATCACCTCAAGATGCCAGGACCCAAGTG(+AAAAAA)GATCCACGTTGAGGTGCATGGACTCTGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606135815_y5zd TTCTGCTTCTTACCCTCCCTTCCAAGCCCTGG(+AAAAAA)GATCCTAAAAGTCCCGCCTTTGTCTGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606135814_snjw CTAGGCAGGACAGACAAAGGCGGGACTTTTAG(+AAAAAA)GATCCCAGGGCTTGGAAGGGAGGGTAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606135813_uqyl GATTCTCTGCTCTTTGTATGACCGGGTGAAAG(+AAAAAA)GTACCTGTCGTCTTTACAAGTTAGTTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240606135811_4wxh ATACAGTCCAAACTAACTTGTAAAGACGACAG(+AAAAAA)GTACCTTTCACCCGGTCATACAAAGAGCAGA NGG 10 8 17 8 24 0 100 5 10