Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135754_a93r CATGTCTCTGCAGAGGCCAGAGGAGGGTATTG(+AAAAAA)GATCCCTAGAAGCTGGAGTTAGAATAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240606135753_dacd GAGACTTACCTATTCTAACTCCAGCTTCTAGG(+AAAAAA)GATCCAATACCCTCCTCTGGCCTCTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606135752_de5o GTTCCAGCAAACAGGAAATGGGGATGCTGAAG(+AAAAAA)GATCCCAGGGAGGGGACGTTACAACCACAGC NGG 10 8 17 8 24 0 100 5 10
    240606135749_j9jl AATCAGCTGTGGTTGTAACGTCCCCTCCCTGG(+AAAAAA)GATCCTTCAGCATCCCCATTTCCTGTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606135747_gfso TGTAACTCTCCCAGGCCCCAGAATAGCTCCTG(+AAAAAA)GATCCAAGCCCGGGGCCCGGCCTGGGACACG NGG 10 8 17 8 24 0 100 5 10
    240606135746_00lc AGCGCCGTGTCCCAGGCCGGGCCCCGGGCTTG(+AAAAAA)GATCCAGGAGCTATTCTGGGGCCTGGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606135743_mxcx CAGTGGGAAGGAGGGGACCTCCCATGCCCAAG(+AAAAAA)GATCCCCAGCGCCAGGGGACAGTGCCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606135741_sro0 AGGCCCCCTGGGCACTGTCCCCTGGCGCTGGG(+AAAAAA)GATCCTTGGGCATGGGAGGTCCCCTCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240606135740_1t5w GGAGGTGACCTCAAGGACTCTGTGGGGATTTG(+AAAAAA)GATCCGGCTGGGCTCCGGCTTGAGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606135739_t123 ATTTTCCAGCCTCTCAAGCCGGAGCCCAGCCG(+AAAAAA)GATCCAAATCCCCACAGAGTCCTTGAGGTCA NGG 10 8 17 8 24 0 100 5 10