Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135653_kajk CCTCACCAGGTAGCTCTCTAGCCCCAAGAAAG(+AAAAAA)GATCCCACCAGAAACAGTATCAGATGTCATA NGG 10 8 17 8 24 0 100 5 10
    240606135651_xa3x TAATTTATGACATCTGATACTGTTTCTGGTGG(+AAAAAA)GATCCTTTCTTGGGGCTAGAGAGCTACCTGG NGG 10 8 17 8 24 0 100 5 10
    240606135649_ng91 CAAGCTGCTGAAACCTTTAAAATGTATCAAGG(+AAAAAA)GATCCATAGCCTCTACCATCCGGAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240606135648_vso3 ATCCTTTAGCATTCCGGATGGTAGAGGCTATG(+AAAAAA)GATCCCTTGATACATTTTAAAGGTTTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606135646_90xd TAATAGTTGCAATGTAATTTATGGAGCACTTG(+AAAAAA)GTACCACATTGTGTCTATTCTTTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606135645_08su CACAAGCAGCTTGAAAGAATAGACACAATGTG(+AAAAAA)GTACCAAGTGCTCCATAAATTACATTGCAAC NGG 10 8 17 8 24 0 100 5 10
    240606135643_5gro ACATTGTCTGTGTACATTCTTTCAAACTCCTG(+AAAAAA)GTACCCTCCACACACTGCTCTGCTTACTATT NGG 10 8 17 8 24 0 100 5 10
    240606135641_7tdn GCAACAATAGTAAGCAGAGCAGTGTGTGGAGG(+AAAAAA)GTACCAGGAGTTTGAAAGAATGTACACAGAC NGG 10 8 17 8 24 0 100 5 10
    240606135639_s805 ATCAGCATGCCTGCCTCATGGGATTAAAGGTG(+AAAAAA)GTACCACCATGCCTGGATCTAATTAAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606135638_o3lt TCTACCCAGCTTAATTAGATCCAGGCATGGTG(+AAAAAA)GTACCACCTTTAATCCCATGAGGCAGGCATG NGG 10 8 17 8 24 0 100 5 10