Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135710_ka64 TCTAGAAGATCCCTGTGCCCCCAGGGGAGGCG(+AAAAAA)GATCCTAAGGGGTGAGGGATAAGGATTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606135708_va8d CCATGCCCCAATCCTTATCCCTCACCCCTTAG(+AAAAAA)GATCCGCCTCCCCTGGGGGCACAGGGATCTT NGG 10 8 17 8 24 0 100 5 10
    240606135706_ew0e AAAAAACTCAGGGTGAGAGGAACGAGGAAGGG(+AAAAAA)GATCCAATTTAGCTCAGCATCCTAAAGTGTT NGG 10 8 17 8 24 0 100 5 10
    240606135705_f72z AAGAAAACACTTTAGGATGCTGAGCTAAATTG(+AAAAAA)GATCCCCTTCCTCGTTCCTCTCACCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240606135703_f5yu CTTTTCAGATAGCCCTGGCTGGTGGGGGAATG(+AAAAAA)GATCCCTGAGAACACGGGCATGTCACACAGG NGG 10 8 17 8 24 0 100 5 10
    240606135702_y8a5 CATTCCCTGTGTGACATGCCCGTGTTCTCAGG(+AAAAAA)GATCCATTCCCCCACCAGCCAGGGCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240606135659_6o6v GCAGGGGGAGAGAGGAGCTGAGGAAATGTGGG(+AAAAAA)GATCCTGGGGGCTCGGACCGGGGTGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606135657_x1x4 ACATAGACACTCACCCCGGTCCGAGCCCCCAG(+AAAAAA)GATCCCCACATTTCCTCAGCTCCTCTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606135655_mu0c CTATAGATGGAGATCTGAAGATTAGACTCTAG(+AAAAAA)GATCCACAGATGAGAGAGTATACGTGACATT NGG 10 8 17 8 24 0 100 5 10
    240606135654_h59g AGGCAAATGTCACGTATACTCTCTCATCTGTG(+AAAAAA)GATCCTAGAGTCTAATCTTCAGATCTCCATC NGG 10 8 17 8 24 0 100 5 10