Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135604_gsqy TCCTTGGGGGTCTGGCAGGTGGCCACACCACG(+AAAAAA)GATCCTTGAGCTGTCTCGAGGGTCAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606135603_hixs TGCCACTGGCCTGACCCTCGAGACAGCTCAAG(+AAAAAA)GATCCGTGGTGTGGCCACCTGCCAGACCCCC NGG 10 8 17 8 24 0 100 5 10
    240606135601_sxca GCTGGCTGCTCTCCTCCCTGCAGTCCACCCTG(+AAAAAA)GATCCCTCGCCTCTAGGGAACAGAAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240606135559_xwhk CCAGCGTAAACTTCTGTTCCCTAGAGGCGAGG(+AAAAAA)GATCCAGGGTGGACTGCAGGGAGGAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606135557_1vd2 CTCTAAGTTGCTCTGACTAGCCTTGAGCCCAG(+AAAAAA)GATCCTCATGAAGGCTGAGATTATAAATATA NGG 10 8 17 8 24 0 100 5 10
    240606135556_inqf GAATTTATATTTATAATCTCAGCCTTCATGAG(+AAAAAA)GATCCTGGGCTCAAGGCTAGTCAGAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240606135554_9acz CACCTCTAACCCCAGCACTGAGGGAATAGGTG(+AAAAAA)GATCCAAGGGGTTCTCTGGACAAGTGGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606135551_b6nu TGGGCTAGACCACTTGTCCAGAGAACCCCTTG(+AAAAAA)GATCCACCTATTCCCTCAGTGCTGGGGTTAG NGG 10 8 17 8 24 0 100 5 10
    240606135550_id4t GGCAGGAAGATGGAGGATTCTTCCAAACATTG(+AAAAAA)GATCCAGGTGACCCTTCTTCCTTGACCATCC NGG 10 8 17 8 24 0 100 5 10
    240606135548_w6du GACAAGGATGGTCAAGGAAGAAGGGTCACCTG(+AAAAAA)GATCCAATGTTTGGAAGAATCCTCCATCTTC NGG 10 8 17 8 24 0 100 5 10