Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135516_hm2o CTGGCAGAGTGCTGGCATCCTCTAAATCCGTG(+AAAAAA)GTACCCAGGGCTACAGGCCCTGAGGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606135513_8gic AGCCTTCCTTGCCTCAGGGCCTGTAGCCCTGG(+AAAAAA)GTACCACGGATTTAGAGGATGCCAGCACTCT NGG 10 8 17 8 24 0 100 5 10
    240606135511_j67f TGGTAAAGAGGTTTAAGATAGTTAATAATGGG(+AAAAAA)GTACCCCAGAAGCAAGAGTGTGTCAGGCAAA NGG 10 8 17 8 24 0 100 5 10
    240606135509_wb6r CTTATTTTGCCTGACACACTCTTGCTTCTGGG(+AAAAAA)GTACCCCATTATTAACTATCTTAAACCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606135507_rdrk GACCATTGTAGCTCATAGTTAAATATGTTAAG(+AAAAAA)GATCCAGCATCTCAAAGATTAAAAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606135506_hh5l TTTTTTTTATTTTTTTAATCTTTGAGATGCTG(+AAAAAA)GATCCTTAACATATTTAACTATGAGCTACAA NGG 10 8 17 8 24 0 100 5 10
    240606135504_3753 TGCAGAGAAGAGGCTAATAATGAACTCAGAGG(+AAAAAA)GTACCTTGAAGACTGGTGTGCCAATGGATAC NGG 10 8 17 8 24 0 100 5 10
    240606135503_955f AACTAGTATCCATTGGCACACCAGTCTTCAAG(+AAAAAA)GTACCCTCTGAGTTCATTATTAGCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606135501_vzxg GTGACGCTCAGATCTGAGAAGAAACATGATGG(+AAAAAA)GATCCATATCAGGTTGTGAATATTTTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606135500_8tmi TGTTCTCAAAAAAATATTCACAACCTGATATG(+AAAAAA)GATCCCATCATGTTTCTTCTCAGATCTGAGC NGG 10 8 17 8 24 0 100 5 10