Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135411_gief TCAACTGACTACGTAGCTGACCTAGAGCTTTG(+AAAAAA)GATCCTCCACCTCCACTTCCCCAGTGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606135409_mxvu TACAGGCCAGCACTGGGGAAGTGGAGGTGGAG(+AAAAAA)GATCCAAAGCTCTAGGTCAGCTACGTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606135408_ict0 CACCATTTGAGGCACAAAATCCAAACTTACAG(+AAAAAA)GATCCGATATTATGTTGATCTGACAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606135407_e0qv AGCCCAGAAACTGTCAGATCAACATAATATCG(+AAAAAA)GATCCTGTAAGTTTGGATTTTGTGCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240606135406_n4io TGCTAAGCAGAATCAGGCTGTAGGAAGGCATG(+AAAAAA)GATCCTGGGGCAGGAACTGCACAGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606135402_tx73 TCAGGGCCTTGGCTGTGCAGTTCCTGCCCCAG(+AAAAAA)GATCCATGCCTTCCTACAGCCTGATTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606135401_xv1g GACGTTGATGGATGACTTTTCCTTCATCCATG(+AAAAAA)GATCCCAAGAGTGGTGCAGTCCGCCAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240606135359_j5pa GCGGTTCTCCTGGCGGACTGCACCACTCTTGG(+AAAAAA)GATCCATGGATGAAGGAAAAGTCATCCATCA NGG 10 8 17 8 24 0 100 5 10
    240606135357_ezla GGTCCAAACAACACATCATCATGATGTCCCAG(+AAAAAA)GATCCACTATGTCCTCATTGCTTCCCAGAAT NGG 10 8 17 8 24 0 100 5 10
    240606135355_pev5 GAAGGATTCTGGGAAGCAATGAGGACATAGTG(+AAAAAA)GATCCTGGGACATCATGATGATGTGTTGTTT NGG 10 8 17 8 24 0 100 5 10