Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135319_x0tg ATGCCCAGGAAAAATCCAGATCTCCATAGCTG(+AAAAAA)GATCCTGGGTGGCTGGCTGGCGCAGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240606135317_7s5y GCCATAGATGCCTGCGCCAGCCAGCCACCCAG(+AAAAAA)GATCCAGCTATGGAGATCTGGATTTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240606135315_z5do GGGAATCTGGTCCCTCCTTGTGAGTTCCTGAG(+AAAAAA)GATCCAACTCAGTTAGGCTTGGTAGCGAGGG NGG 10 8 17 8 24 0 100 5 10
    240606135314_h96n GAAGGCCCTCGCTACCAAGCCTAACTGAGTTG(+AAAAAA)GATCCTCAGGAACTCACAAGGAGGGACCAGA NGG 10 8 17 8 24 0 100 5 10
    240606135313_iuk8 GCTGTTTTCAGACACACCAGAAGAGGGCATCG(+AAAAAA)GATCCCATCACACATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240606135311_70c3 ACCACATGGTGGCTCACAACCATGTGTGATGG(+AAAAAA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240606135309_3tui GGCTCACAATGCCTGTACCTCCAGCTTTGAGG(+AAAAAA)GATCCTTTATTCAGGTGTACTTGGACACATG NGG 10 8 17 8 24 0 100 5 10
    240606135308_6x6j GTCTACATGTGTCCAAGTACACCTGAATAAAG(+AAAAAA)GATCCCTCAAAGCTGGAGGTACAGGCATTGT NGG 10 8 17 8 24 0 100 5 10
    240606135307_pbv0 TGATTTCTGTGGGGGCTCAGAAGAGGGCACTG(+AAAAAA)GATCCCTGGAGCTGGAGTTACAGATGGCCGT NGG 10 8 17 8 24 0 100 5 10
    240606135305_o2n5 AGCTCACGGCCATCTGTAACTCCAGCTCCAGG(+AAAAAA)GATCCAGTGCCCTCTTCTGAGCCCCCACAGA NGG 10 8 17 8 24 0 100 5 10