Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135429_2h0m GCATGTATTCAGTTTTAGAATCACTTTTACTG(+AAAAAA)GATCCTGTAGTAATGTCTAGAAGTATCATAT NGG 10 8 17 8 24 0 100 5 10
    240606135427_3be1 AATAGATATGATACTTCTAGACATTACTACAG(+AAAAAA)GATCCAGTAAAAGTGATTCTAAAACTGAATA NGG 10 8 17 8 24 0 100 5 10
    240606135425_ou35 TTACTTGAAAATTGGACATAGTACTACCAGAG(+AAAAAA)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606135423_tclp TTCTGGATATATGCCCAGGAGAGGTATTGTGG(+AAAAAA)GATCCTCTGGTAGTACTATGTCCAATTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606135420_9u9g GGATGGAGCATCTAGAGACTGTCATACCCGGG(+AAAAAA)GATCCATCCCATTATTAGCTCCAAACGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606135418_d6mg TGGTTTCAGCGTTTGGAGCTAATAATGGGATG(+AAAAAA)GATCCCCGGGTATGACAGTCTCTAGATGCTC NGG 10 8 17 8 24 0 100 5 10
    240606135417_nzj7 ACTCATAACCTAGCTAAGAATTTTTGAGTCTG(+AAAAAA)GTACCCATGGAGCACAAAAGCATTACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606135415_dfjh ATACATTTCTGTAATGCTTTTGTGCTCCATGG(+AAAAAA)GTACCAGACTCAAAAATTCTTAGCTAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606135413_5s63 AGACTCAGGAAAGAGGTACTTGGGGTCTGTGG(+AAAAAA)GTACCCAGATATATCTAAAAATACATAGACA NGG 10 8 17 8 24 0 100 5 10
    240606135412_b7vf CCAGCTGTCTATGTATTTTTAGATATATCTGG(+AAAAAA)GTACCCACAGACCCCAAGTACCTCTTTCCTG NGG 10 8 17 8 24 0 100 5 10