Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606135810_qju3 TATAAGTTTAGTATACAATGGATCAAATTAAG(+AAAAAA)GATCCTGCTCTCTTTCCTTTGGAGGTAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606135809_30y7 ACGTCGAACTACCTCCAAAGGAAAGAGAGCAG(+AAAAAA)GATCCTTAATTTGATCCATTGTATACTAAAC NGG 10 8 17 8 24 0 100 5 10
    240606135807_wxxj CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AAAAAA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606135805_gfc6 GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AAAAAA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606135804_xd2b CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AAAAAA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606135802_pt3b GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AAAAAA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606135800_6p5z CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AAAAAA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606135759_l1fe GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AAAAAA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606135757_nrq6 CCTGCAGCCACCTAAGAGTGTAGGTGTTCCAG(+AAAAAA)GATCCTCCTCTGGGGATATCTTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606135756_g7ew GGGTCTCTTTCTCTAAGATATCCCCAGAGGAG(+AAAAAA)GATCCTGGAACACCTACACTCTTAGGTGGCT NGG 10 8 17 8 24 0 100 5 10