Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140826_g3th TCATGAGAGGAAAGACTCCCCATTTACAGGAG(+AAAAAA)GTACCTCATTTGTCAAAGAATGAAGGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240606140823_fndl GTCCAGAGCCTGTTTCCTCAGGGCTCCCCTGG(+AAAAAA)GTACCCAGCTCCCAGGGTAGGTGCCCACCTG NGG 10 8 17 8 24 0 100 5 10
    240606140822_x8eu GAGTTCAGGTGGGCACCTACCCTGGGAGCTGG(+AAAAAA)GTACCCAGGGGAGCCCTGAGGAAACAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606140820_p0c3 CTTGGATGTTGGGAGTGTAGCAGGCACAGGAG(+AAAAAA)GTACCTCTGCAGAAGGAAGGCGGAGGGTGGA NGG 10 8 17 8 24 0 100 5 10
    240606140818_mnc0 AGGCCTCCACCCTCCGCCTTCCTTCTGCAGAG(+AAAAAA)GTACCTCCTGTGCCTGCTACACTCCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240606140817_2bkd TGTCCGTTTCCCCTCACTGTCAGGGAAAGCTG(+AAAAAA)GATCCCTCTGGCTGACTTAGAGTACTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240606140815_ludm CCCAAGTCACAGTACTCTAAGTCAGCCAGAGG(+AAAAAA)GATCCAGCTTTCCCTGACAGTGAGGGGAAAC NGG 10 8 17 8 24 0 100 5 10
    240606140813_vnbp GCCGCTGGCTGGGTGGCCCAGGGGGGCTGGCG(+AAAAAA)GTACCGCCATGCTGGTTCCTTGGGGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606140811_pmh4 TTCCAGAGGCCCCCCAAGGAACCAGCATGGCG(+AAAAAA)GTACCGCCAGCCCCCCTGGGCCACCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606140810_k3j6 GAGCCAGAGTTGGGGATCTGAGCCCATGAATG(+AAAAAA)GTACCGCTTAGACCTCAGGCCAAAACACACC NGG 10 8 17 8 24 0 100 5 10