Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140857_bjby GAATAAAATGTCCTGCTAGTGCTCTGAAGTAG(+AAAAAA)GTACCAGCTGATTTTCCAGATGTTGAAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606140856_4nid TGTCCACTATATAACAGGGTGTCATGGACAAG(+AAAAAA)GATCCTCTTAGTTATTTGCTTTGAATGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606140855_w5vi TCAAGTTCACATTCAAAGCAAATAACTAAGAG(+AAAAAA)GATCCTTGTCCATGACACCCTGTTATATAGT NGG 10 8 17 8 24 0 100 5 10
    240606140854_n2nc AGGAATTGACTGTTATATTAAGACTTCTAAAG(+AAAAAA)GATCCCTGCCCTGGAGTAATTTCAGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606140852_qi7i GTCTGACAGCACTGAAATTACTCCAGGGCAGG(+AAAAAA)GATCCTTTAGAAGTCTTAATATAACAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606140850_87z8 TTTCTGCACTGTAAGTTATTTACAAAGTTGAG(+AAAAAA)GATCCTTTGATGGAGGAAAAAAAACCCACGT NGG 10 8 17 8 24 0 100 5 10
    240606140849_fswj GAGAAACGTGGGTTTTTTTTCCTCCATCAAAG(+AAAAAA)GATCCTCAACTTTGTAAATAACTTACAGTGC NGG 10 8 17 8 24 0 100 5 10
    240606140847_udo8 GGTGGCACTTCCTGCCAGGACCTCCCTAATGG(+AAAAAA)GTACCTGGCAGAAGAGGTAGGTTACCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240606140845_txmq AACTGACCCAGGTAACCTACCTCTTCTGCCAG(+AAAAAA)GTACCCATTAGGGAGGTCCTGGCAGGAAGTG NGG 10 8 17 8 24 0 100 5 10
    240606140844_9agg TCCTTGAGAAACACATTTGGGAAGTTTCTTCG(+AAAAAA)GATCCTTCTTTCTGAGCTCAAGGAAGAAAAT NGG 10 8 17 8 24 0 100 5 10