Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606140809_vug9 GGGAGGGTGTGTTTTGGCCTGAGGTCTAAGCG(+AAAAAA)GTACCATTCATGGGCTCAGATCCCCAACTCT NGG 10 8 17 8 24 0 100 5 10
    240606140807_lgts CCAATCAGACCTAGATCCTGGCTGCTCTAGTG(+AAAAAA)GTACCAAGGTGTCCTGAGATATGGATGTTGT NGG 10 8 17 8 24 0 100 5 10
    240606140805_fmtu TGCACACAACATCCATATCTCAGGACACCTTG(+AAAAAA)GTACCACTAGAGCAGCCAGGATCTAGGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606140804_zm7v GTGTTATATGTTAAAACTTTCTCTTTAGCAAG(+AAAAAA)GTACCAAGAAATTCACCTGTTCCTTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240606140802_290o GCCCATATCTGAAGGAACAGGTGAATTTCTTG(+AAAAAA)GTACCTTGCTAAAGAGAAAGTTTTAACATAT NGG 10 8 17 8 24 0 100 5 10
    240606140801_t964 AGATTATATAGAAACAATCTGTACTGCTCTGG(+AAAAAA)GATCCTTTGGTCTTAGAAACCATTTTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606140759_873s CACCCCCCCAAAAATGGTTTCTAAGACCAAAG(+AAAAAA)GATCCCAGAGCAGTACAGATTGTTTCTATAT NGG 10 8 17 8 24 0 100 5 10
    240606140758_1xb5 TTCATGATTTCTTATCATGTTACCATAGCAAG(+AAAAAA)GATCCAGGTCCTTGCTGGAGTCAGTGGTCAT NGG 10 8 17 8 24 0 100 5 10
    240606140756_r6uf GTCACATGACCACTGACTCCAGCAAGGACCTG(+AAAAAA)GATCCTTGCTATGGTAACATGATAAGAAATC NGG 10 8 17 8 24 0 100 5 10
    240606140755_fxk6 ATTAGTGTAAAGGTATCTCACCCTTAGATCTG(+AAAAAA)GATCCCTTGGAACTGGAGTTACAATTGTAAG NGG 10 8 17 8 24 0 100 5 10