Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141020_9iez TTAGTTACTACCAACCAGGGCTTTATCCTTAG(+AAAAAA)GATCCCAAGGCGTGAAAGGTAAAGAGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606141019_knnb AAAACGGCAGCACCTCACGGCTGTCTTTCTAG(+AAAAAA)GATCCTCCATTATTCCTTAGAAGCAAGGACC NGG 10 8 17 8 24 0 100 5 10
    240606141017_9idp GACCAGGTCCTTGCTTCTAAGGAATAATGGAG(+AAAAAA)GATCCTAGAAAGACAGCCGTGAGGTGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606141016_2z80 GGCCCGTGTAGTTGGCTCAGTGTCACACAACG(+AAAAAA)GTACCAGAGCACCATGATGCCTTTCTGTCTG NGG 10 8 17 8 24 0 100 5 10
    240606141015_vl2c GCGGTCAGACAGAAAGGCATCATGGTGCTCTG(+AAAAAA)GTACCGTTGTGTGACACTGAGCCAACTACAC NGG 10 8 17 8 24 0 100 5 10
    240606141013_qsxr CACCCTTGGGCCTACCTGTTTGCCTGAGGTGG(+AAAAAA)GATCCCAGGAGCCTCACCCCTACCACCCCTC NGG 10 8 17 8 24 0 100 5 10
    240606141011_7qit ACATGGAGGGGTGGTAGGGGTGAGGCTCCTGG(+AAAAAA)GATCCCACCTCAGGCAAACAGGTAGGCCCAA NGG 10 8 17 8 24 0 100 5 10
    240606141009_pbfl GAGAGGAGGCCCTTGCCCTAGGCTGTGAGTAG(+AAAAAA)GTACCCAGCTGGGCACAACTCAGGCTTCATC NGG 10 8 17 8 24 0 100 5 10
    240606141008_f5pa AAAAGGATGAAGCCTGAGTTGTGCCCAGCTGG(+AAAAAA)GTACCTACTCACAGCCTAGGGCAAGGGCCTC NGG 10 8 17 8 24 0 100 5 10
    240606141006_bx4f ACACCAGGCTCCCCACGAGTTCCCATTGTGGG(+AAAAAA)GATCCTGGCAGGATTCCTTTCTCTAAAATGG NGG 10 8 17 8 24 0 100 5 10