Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141257_jltt TTAAGTGGATTGACAATGACAGAACATGTCTG(+AAAAAA)GATCCATCCTTAGACATGCTTTTGCTGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606141256_tio5 TATCAACTTCAGCAAAAGCATGTCTAAGGATG(+AAAAAA)GATCCAGACATGTTCTGTCATTGTCAATCCA NGG 10 8 17 8 24 0 100 5 10
    240606141255_phgm GGGCCACTTCCTGTCCACTTGACAGCCTGCAG(+AAAAAA)GTACCGCACTGGCAAAGCTTTGGCCCCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606141254_arm5 CCCATCTCTGGGGCCAAAGCTTTGCCAGTGCG(+AAAAAA)GTACCTGCAGGCTGTCAAGTGGACAGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606141252_63yg GAGGGAGGGGGTATATGTAGCAAGTGTGCTTG(+AAAAAA)GATCCTGGTTAGGGCAGGGCCACTTCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240606141251_c06n AAGTGGACAGGAAGTGGCCCTGCCCTAACCAG(+AAAAAA)GATCCAAGCACACTTGCTACATATACCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606141249_8maa GGTTGGGGGCTCTGCCATCCCTCCCCTGCTGG(+AAAAAA)GTACCCTGTTACCATTCATTGTCTGTGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606141248_a09c GGGAAACAGCACAGACAATGAATGGTAACAGG(+AAAAAA)GTACCCAGCAGGGGAGGGATGGCAGAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240606141246_f81f ACACTGCCTGTGCCCCAACTCTGCCTCACTCG(+AAAAAA)GATCCGTCTGCCCTGTGGGGACATGAGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606141245_cvvc ACAGAGACACTCATGTCCCCACAGGGCAGACG(+AAAAAA)GATCCGAGTGAGGCAGAGTTGGGGCACAGGC NGG 10 8 17 8 24 0 100 5 10