Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141344_l24y AATGAAATGGCCCAAAGTGAGCTCACTGCTGG(+AAAAAA)GATCCTGGCAGGCTACCTAGACCCCACGGTC NGG 10 8 17 8 24 0 100 5 10
    240606141343_bd74 AAGGCTGAGATTAAAGAAAGGTTCCTGCTGTG(+AAAAAA)GTACCCAACTTTTTATCTGGGTGCTGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240606141341_jxu7 CACGGGGACACAGCACCCAGATAAAAAGTTGG(+AAAAAA)GTACCACAGCAGGAACCTTTCTTTAATCTCA NGG 10 8 17 8 24 0 100 5 10
    240606141340_4py7 CATGTAGAACACAAGAAGACCCTTTTCTTCTG(+AAAAAA)GATCCTCTGCTAGCTACCATCTAAACTGTCC NGG 10 8 17 8 24 0 100 5 10
    240606141339_dtq8 AAATGGGACAGTTTAGATGGTAGCTAGCAGAG(+AAAAAA)GATCCAGAAGAAAAGGGTCTTCTTGTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240606141337_9wb1 TTGGCTAAACTGACTGGCATGCGAGCACCAGG(+AAAAAA)GATCCTCCTGTCTCTGCCTTCCAAAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240606141335_wg2w TAATCTCAGCCTTTGGAAGGCAGAGACAGGAG(+AAAAAA)GATCCCTGGTGCTCGCATGCCAGTCAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606141333_8yro CCCCCACAGCCCTTTGATGTTACCAGGCACCG(+AAAAAA)GATCCCCATGGCTTCCGTCACCCCTGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606141331_hz7a TGGTAAGGACCAGGGGTGACGGAAGCCATGGG(+AAAAAA)GATCCGGTGCCTGGTAACATCAAAGGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606141330_ketd TATTTTTCCCTTTCAAATAAAACACCAGTCAG(+AAAAAA)GTACCTTTATCCCAGTCTTAACTGTCCCAAA NGG 10 8 17 8 24 0 100 5 10