Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141416_8dh0 GGCTGGTCAACTGTGCGCCTTCAGTGTAGCAG(+AAAAAA)GATCCTGGTGAATCAGTCCATCTACAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240606141415_4yvj GTGATTAGAGTAGGTGGTCGGGGCCACACTAG(+AAAAAA)GTACCTAACCTACACACAAGCACACAGAATC NGG 10 8 17 8 24 0 100 5 10
    240606141414_3c0t TGCCAGATTCTGTGTGCTTGTGTGTAGGTTAG(+AAAAAA)GTACCTAGTGTGGCCCCGACCACCTACTCTA NGG 10 8 17 8 24 0 100 5 10
    240606141412_8eqy GTTCTAAGGGGAAAATATCCTTTGAGAATTGG(+AAAAAA)GTACCATAGTGACAAAGCCTTGACAACAGGA NGG 10 8 17 8 24 0 100 5 10
    240606141411_7ysf CCCATTCCTGTTGTCAAGGCTTTGTCACTATG(+AAAAAA)GTACCCAATTCTCAAAGGATATTTTCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240606141409_uslq AGGAGGGCCAGGCAGGGAGGGTCCCTAGAGGG(+AAAAAA)GATCCCTCAGCCCTCGAGGCTGCAGCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606141408_fimx GTCAATGGCTGCTGCAGCCTCGAGGGCTGAGG(+AAAAAA)GATCCCCTCTAGGGACCCTCCCTGCCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240606141406_w6h3 TATAGGTGATGGGGGGGTCACAGATGGCACAG(+AAAAAA)GTACCCCAGTCTTATCCAGCTCTGCTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240606141404_529a AAAGGGGCAGAGCAGAGCTGGATAAGACTGGG(+AAAAAA)GTACCTGTGCCATCTGTGACCCCCCCATCAC NGG 10 8 17 8 24 0 100 5 10
    240606141403_zj26 GGCACTGACTGCCTCAGCCCTCAGCATCTAAG(+AAAAAA)GTACCATGGTCAGCATGGCCAAAAGTCCCCT NGG 10 8 17 8 24 0 100 5 10