Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141555_52mo CCCAAAATAGTTACCAGCCATGGGCCACGTAG(+AAAAAA)GTACCTGTGGTATTCATAGAGTTTCAACAAA NGG 10 8 17 8 24 0 100 5 10
    240606141553_hqfd CCAGAGAGTGACCCGGTTGCCAACCATAGAGG(+AAAAAA)GTACCTATGATTCTTCATTTCTCAGGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240606141552_cq7i TAAAGGCAAACCTGAGAAATGAAGAATCATAG(+AAAAAA)GTACCCTCTATGGTTGGCAACCGGGTCACTC NGG 10 8 17 8 24 0 100 5 10
    240606141551_rwk3 AATAAATAAAGTGTAGTGATTCATTAAATATG(+AAAAAA)GTACCTACAGGATCAAAAATACCAGTACTCC NGG 10 8 17 8 24 0 100 5 10
    240606141549_5ug1 TGATTGGAGTACTGGTATTTTTGATCCTGTAG(+AAAAAA)GTACCATATTTAATGAATCACTACACTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606141548_ebrf TGCTTAGGAGTGAGAACTGCCAGGGTAGCCAG(+AAAAAA)GATCCTCTCACAGTATTAAGGCGTGCCCACA NGG 10 8 17 8 24 0 100 5 10
    240606141547_ldx8 TCCCATGTGGGCACGCCTTAATACTGTGAGAG(+AAAAAA)GATCCTGGCTACCCTGGCAGTTCTCACTCCT NGG 10 8 17 8 24 0 100 5 10
    240606141545_43nr GCCCTCATCTCTGCTACCGTTTACATCATCCG(+AAAAAA)GTACCTTTGTAGCCTTAGACCGATGATCACC NGG 10 8 17 8 24 0 100 5 10
    240606141543_39x3 AACACGGTGATCATCGGTCTAAGGCTACAAAG(+AAAAAA)GTACCGGATGATGTAAACGGTAGCAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240606141542_x4fx CCAACACTTAGAACATTTTTTTAAACAGAAAG(+AAAAAA)GTACCAGTACCTAGACACAAACGTTAATGAG NGG 10 8 17 8 24 0 100 5 10