Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141624_z4fd CATGAGTGGTTAGTTCCTAGTGGCTGTGGATG(+AAAAAA)GTACCAGCACTAACTACTTCTCTACTGTGGT NGG 10 8 17 8 24 0 100 5 10
    240606141623_ybc3 CTCAGTGCTGAAAGACTTGATTTTGTAGCTTG(+AAAAAA)GTACCTCAGCGTTGACTTAGGTGCTCCACTA NGG 10 8 17 8 24 0 100 5 10
    240606141622_2788 TGGGCTAGTGGAGCACCTAAGTCAACGCTGAG(+AAAAAA)GTACCAAGCTACAAAATCAAGTCTTTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606141621_f1h3 ATGCCCTCTTTCATGGAGCTGTTCGCACTGCG(+AAAAAA)GATCCGATTCCTCGGGCTTGGATTCCACTCC NGG 10 8 17 8 24 0 100 5 10
    240606141619_9w41 TCTCGGGAGTGGAATCCAAGCCCGAGGAATCG(+AAAAAA)GATCCGCAGTGCGAACAGCTCCATGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240606141618_hu8a TCCCTGGCCTGGGCGCCCCTTGCTGTTGGGAG(+AAAAAA)GATCCCATCCTCGAGTGCCTCCTATCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606141617_tz5n AAGGCAGGAGGATAGGAGGCACTCGAGGATGG(+AAAAAA)GATCCTCCCAACAGCAAGGGGCGCCCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240606141615_zt8i TGACTTCTGAAACGAAGAGAAAAGCACCTATG(+AAAAAA)GATCCCACTCCTTCCCATCGCTTACCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606141613_rucs CTGGGGAACAGGTAAGCGATGGGAAGGAGTGG(+AAAAAA)GATCCATAGGTGCTTTTCTCTTCGTTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240606141612_4t9y TAACCCATGAACTAGCTGTAACATAGTTTGTG(+AAAAAA)GATCCATGCACTGATGCTGTGAAGCCTGCCC NGG 10 8 17 8 24 0 100 5 10