Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606141756_6hi9 AGGTGGCTTTCGGGCTACCATGCTCCCTCATG(+AAAAAA)GATCCAGCTCTCCCACCCTGTCACCTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606141755_2329 CTCTCTCCAGTTAGTACCTATCTGGGATATTG(+AAAAAA)GTACCTTTGGATATGAATGTCCTTATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240606141753_i2iw ATATTTTCTTATAAGGACATTCATATCCAAAG(+AAAAAA)GTACCAATATCCCAGATAGGTACTAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240606141752_48to TGAAACTGGACTTGCTGGGGTGTCGCTAAGAG(+AAAAAA)GATCCCATTTGACCTCTGCCTTGCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240606141750_lphx TCTCAGGCTGTGGCAAGGCAGAGGTCAAATGG(+AAAAAA)GATCCTCTTAGCGACACCCCAGCAAGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606141749_kbkv CTTTGCTGCCTAAGAACCGGAGCCAACAGCAG(+AAAAAA)GTACCTCCTGGGGTTCCCTGAGGAGTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606141747_0n0b GTTTTAGCACACTCCTCAGGGAACCCCAGGAG(+AAAAAA)GTACCTGCTGTTGGCTCCGGTTCTTAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240606141745_yc4m GTAGGGTCCCAAAGGAGAGCCGGGGCAGGGTG(+AAAAAA)GATCCCTGCTTTGCTGCCTAAGAACCGGAGC NGG 10 8 17 8 24 0 100 5 10
    240606141744_pl6l TGTTGGCTCCGGTTCTTAGGCAGCAAAGCAGG(+AAAAAA)GATCCACCCTGCCCCGGCTCTCCTTTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240606141742_o853 GGCAGAGGGACCTATGGCCAGATGCCAGGGAG(+AAAAAA)GTACCCAGAGACCCCACAGCGTGGTTCATGC NGG 10 8 17 8 24 0 100 5 10