Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133602_9lo8 ACCGAGGCTCATTTTGGCATTGTACAAAGGAG(+AAAAAA)GTACCATTAGGATGCTTGATGTCCAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240606133600_bcaa CTGGAGTTGGTCATTTTTGTACACCCACAGTG(+AAAAAA)GTACCTTTTACAGTCAGGTTTGGATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240606133559_ol05 AGCTGCAAAGTATCCAAACCTGACTGTAAAAG(+AAAAAA)GTACCACTGTGGGTGTACAAAAATGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240606133557_4yys CCCCCAGTTCTCTGGCTGAGGCAACATTCCTG(+AAAAAA)GTACCAGTGCCTATCACTTTGTTTCAGGACT NGG 10 8 17 8 24 0 100 5 10
    240606133556_dt6u TAGGCAGTCCTGAAACAAAGTGATAGGCACTG(+AAAAAA)GTACCAGGAATGTTGCCTCAGCCAGAGAACT NGG 10 8 17 8 24 0 100 5 10
    240606133555_2b10 GTGTGTGTGTGTGTGTGTGTGTGTACTCTAGG(+AAAAAA)GTACCTCAGTATGTCCTGAAAGTTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240606133553_nlxl AAAACTCTGTTGAACTTTCAGGACATACTGAG(+AAAAAA)GTACCCTAGAGTACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240606133552_849g GAAAAGCACCATGGGAAATCCCTTGGCTTCTG(+AAAAAA)GTACCAGCATGGTAATTGCAAGGGAAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240606133551_a0r1 TCTGAATTTCTTCCCTTGCAATTACCATGCTG(+AAAAAA)GTACCAGAAGCCAAGGGATTTCCCATGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606133550_j720 AGAACTTGGGTCTGCTTTCTACAGTCATTATG(+AAAAAA)GTACCAAAACCATATGATTCTTTGTACAGTT NGG 10 8 17 8 24 0 100 5 10