Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606134653_qvba CCTCGTATACTCCAGGACCAATCAGAACACAG(+AAAAAA)GTACCAGGCAGAATCTAATTCAAACGATTTT NGG 10 8 17 8 24 0 100 5 10
    240606134652_ljiu TGAAAGCATTACTATATTTTTTACATTAAGTG(+AAAAAA)GTACCAATAAAGTATTTTGTTACCAAAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606134651_fprv CATTTAGCTTTTGGTAACAAAATACTTTATTG(+AAAAAA)GTACCACTTAATGTAAAAAATATAGTAATGC NGG 10 8 17 8 24 0 100 5 10
    240606134649_2tbx CTTCAAAGTCACGGGCCAGCCAGACACAAAAG(+AAAAAA)GTACCAGTCAAAGTCGCATTGTGGGAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240606134648_t6qd ACAGTCTAGCTCCCACAATGCGACTTTGACTG(+AAAAAA)GTACCTTTTGTGTCTGGCTGGCCCGTGACTT NGG 10 8 17 8 24 0 100 5 10
    240606134647_j2n6 GTTTCGGAAGTTGATTCATGTAGCTGACAGTG(+AAAAAA)GATCCAGAAGGGTCAGTCCCCTTCACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606134646_mvp0 ACAAGATGGAGTGAAGGGGACTGACCCTTCTG(+AAAAAA)GATCCACTGTCAGCTACATGAATCAACTTCC NGG 10 8 17 8 24 0 100 5 10
    240606134644_cf4u ATAGTACCCATGGTGTGTTCTGATCGATCCTG(+AAAAAA)GATCCCCGGACTCCTTGGGACATGCTATTGA NGG 10 8 17 8 24 0 100 5 10
    240606134641_n3pk CTGCTTCAATAGCATGTCCCAAGGAGTCCGGG(+AAAAAA)GATCCAGGATCGATCAGAACACACCATGGGT NGG 10 8 17 8 24 0 100 5 10
    240606134640_kldy AAGCCCCTCTTGTTCCTCCATCACTAAAAGAG(+AAAAAA)GATCCCTGAAATCAGACCAAAGCCACACATC NGG 10 8 17 8 24 0 100 5 10