Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133549_pqjg GGATAAACTGTACAAAGAATCATATGGTTTTG(+AAAAAA)GTACCATAATGACTGTAGAAAGCAGACCCAA NGG 10 8 17 8 24 0 100 5 10
    240606133548_f20q AGCTTGTTTCTGAGAAAACCAAGTTCTAAGTG(+AAAAAA)GTACCCAAAGGTGTTGCCAGTCCTTCCTATG NGG 10 8 17 8 24 0 100 5 10
    240606133546_jw2u GGATGCATAGGAAGGACTGGCAACACCTTTGG(+AAAAAA)GTACCACTTAGAACTTGGTTTTCTCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240606133545_pwt4 CTCCATGGTTAAGAACACTAGTTTTTGCTGAG(+AAAAAA)GATCCCAGTTCAATTCCCAGTACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240606133543_rpts AGCCACCATGTGGGTACTGGGAATTGAACTGG(+AAAAAA)GATCCTCAGCAAAAACTAGTGTTCTTAACCA NGG 10 8 17 8 24 0 100 5 10
    240606133542_qfvr CTGGATGCCGGTGATGCTGGAGTAGACGGCTG(+AAAAAA)GATCCCACCACGCACATTTCACGTTATTTGA NGG 10 8 17 8 24 0 100 5 10
    240606133540_xx7b ACTAATCAAATAACGTGAAATGTGCGTGGTGG(+AAAAAA)GATCCAGCCGTCTACTCCAGCATCACCGGCA NGG 10 8 17 8 24 0 100 5 10
    240606133539_lddn GATCACTCTGAAGGTCTGATTGTCCTCTCTTG(+AAAAAA)GATCCCCTTGAGGAAGAGTAACCCTGTCACA NGG 10 8 17 8 24 0 100 5 10
    240606133537_ra7u TGCTGTGTGACAGGGTTACTCTTCCTCAAGGG(+AAAAAA)GATCCAAGAGAGGACAATCAGACCTTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606133536_nltk CATCAGTTTAGGGATGGCCAGGCCATAGTGAG(+AAAAAA)GTACCTGACCAGATCTCAGAGTCAGGCTCCC NGG 10 8 17 8 24 0 100 5 10