Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133433_0nex GCTTACAACTGTCTGTAACTCCAGTGTCAGGG(+AAAAAA)GATCCAACACTCACATAGTCACACACACTGG NGG 10 8 17 8 24 0 100 5 10
    240606133432_01jj GAATGTAAACAGTTTTATTCAGAAACAGATAG(+AAAAAA)GTACCTGTTTGCATTACAGAATATAAAACTT NGG 10 8 17 8 24 0 100 5 10
    240606133431_ktp0 AAACCAAGTTTTATATTCTGTAATGCAAACAG(+AAAAAA)GTACCTATCTGTTTCTGAATAAAACTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240606133429_d92h GGAGAAGAGTGGTTTGCCTTCTGAGGTATGAG(+AAAAAA)GATCCCTGGTCCAATCAGCAGGGTGAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240606133427_xwsz TTCCTAAACCTCACCCTGCTGATTGGACCAGG(+AAAAAA)GATCCTCATACCTCAGAAGGCAAACCACTCT NGG 10 8 17 8 24 0 100 5 10
    240606133426_sot9 ACCTGGCTGGAAGGGCACACTTCAGGGCTGAG(+AAAAAA)GATCCAGTCTGGTCCTGCAGCCTGTTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240606133424_oa7m CATAATGGCAAACAGGCTGCAGGACCAGACTG(+AAAAAA)GATCCTCAGCCCTGAAGTGTGCCCTTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606133423_yyw9 ACCACACATATGTAATTCAGAGAACACTTCCG(+AAAAAA)GATCCTTCACCCTCCCTCTAACACATCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606133422_5pde AGGGTAGAGGATGTGTTAGAGGGAGGGTGAAG(+AAAAAA)GATCCGGAAGTGTTCTCTGAATTACATATGT NGG 10 8 17 8 24 0 100 5 10
    240606133419_94bz CACTTTCCTCCCCCCAAAACCTGGCCCCAAGG(+AAAAAA)GTACCCCCAAAACAAAAAAAAAGAAAGAAAA NGG 10 8 17 8 24 0 100 5 10