Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133344_alox CAATTTTGCATGATTTATGGTGTTACCAGATG(+AAAAAA)GATCCTCTCTTGTGCTACCTCTGAGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240606133343_a8kh ATCATAATTCTTTTTAATTTTAAAATAGTCAG(+AAAAAA)GATCCAGTTTGAAAATATGCCACGTTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240606133342_i156 TCTCTTTTTAAACGTGGCATATTTTCAAACTG(+AAAAAA)GATCCTGACTATTTTAAAATTAAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240606133340_1763 TGGTGTTGGACGTCGGAAGTCCTCCTGTAGTG(+AAAAAA)GATCCAAAACTCACCTCCTTATGAAAGACAA NGG 10 8 17 8 24 0 100 5 10
    240606133339_eenk CCATGTTGTCTTTCATAAGGAGGTGAGTTTTG(+AAAAAA)GATCCACTACAGGAGGACTTCCGACGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606133338_t0q3 ATATGAGGCGCAGTTGCTTTCTATAGGACATG(+AAAAAA)GATCCTCATACTCAGTCATTTCATGAACCCT NGG 10 8 17 8 24 0 100 5 10
    240606133337_5it0 TCTCAAGGGTTCATGAAATGACTGAGTATGAG(+AAAAAA)GATCCATGTCCTATAGAAAGCAACTGCGCCT NGG 10 8 17 8 24 0 100 5 10
    240606133336_9sv6 CGAATTCCTTTACTGATGACCTACCTGAGCTG(+AAAAAA)GATCCTCAGCATCCACATCAAGGAAGAAAAG NGG 10 8 17 8 24 0 100 5 10
    240606133334_kvpo TGGTTCTTTTCTTCCTTGATGTGGATGCTGAG(+AAAAAA)GATCCAGCTCAGGTAGGTCATCAGTAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240606133333_7rav TTACTTTAGCATTTTGCATTAAGATTCAAAAG(+AAAAAA)GTACCTTTTCTCTCTTTAGTGCTGTTGTAAA NGG 10 8 17 8 24 0 100 5 10