Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133332_alkl TAGGCTTTACAACAGCACTAAAGAGAGAAAAG(+AAAAAA)GTACCTTTTGAATCTTAATGCAAAATGCTAA NGG 10 8 17 8 24 0 100 5 10
    240606133330_0pik GATGTTCTGGCTAATGTACACAAGGAGGTGTG(+AAAAAA)GATCCAGCAAGTGGTTTCTTCTTTGTTTTAT NGG 10 8 17 8 24 0 100 5 10
    240606133329_ykzl CAATAATAAAACAAAGAAGAAACCACTTGCTG(+AAAAAA)GATCCACACCTCCTTGTGTACATTAGCCAGA NGG 10 8 17 8 24 0 100 5 10
    240606133328_ezrh ATCATGTAATTTATCTGCTCAAAACCCTCTGG(+AAAAAA)GTACCCCTATGCAGTCAAAACACTGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606133326_chcx CTAAAAGGAAGCAGTGTTTTGACTGCATAGGG(+AAAAAA)GTACCCAGAGGGTTTTGAGCAGATAAATTAC NGG 10 8 17 8 24 0 100 5 10
    240606133324_9862 TCATGGATGGTACTCTGGTTTTATTTGGAATG(+AAAAAA)GTACCTGATTTTCACTCACCATTATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240606133323_oot0 GGCAGAATGAGATAATGGTGAGTGAAAATCAG(+AAAAAA)GTACCATTCCAAATAAAACCAGAGTACCATC NGG 10 8 17 8 24 0 100 5 10
    240606133322_34zf CATGGCAGGTGGCTCTAAGGACACCTAGCATG(+AAAAAA)GTACCCATTTGATTAAGAGAAGAAGATGCCT NGG 10 8 17 8 24 0 100 5 10
    240606133320_41ib CAGGAAGGCATCTTCTTCTCTTAATCAAATGG(+AAAAAA)GTACCATGCTAGGTGTCCTTAGAGCCACCTG NGG 10 8 17 8 24 0 100 5 10
    240606133319_i62a TTAGGCTGTTTGGCAGATACAACAGAGTCCTG(+AAAAAA)GATCCTCCTTGCCTCCTTCAGAAGCTCCGTA NGG 10 8 17 8 24 0 100 5 10