Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133302_y9j8 CAGGATCTGATACCTTCCTCTGGACTCTGAAG(+AAAAAA)GTACCGCCACACACACTTAGCATACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240606133301_o5io CATACACTATTCCCGAGTTAGTCTGGTGTATG(+AAAAAA)GATCCACGTGCTCTCTTTTAGTATTATCTTA NGG 10 8 17 8 24 0 100 5 10
    240606133300_v0l7 TATAATAAGATAATACTAAAAGAGAGCACGTG(+AAAAAA)GATCCATACACCAGACTAACTCGGGAATAGT NGG 10 8 17 8 24 0 100 5 10
    240606133258_mssu TGCCCCATAGACCCGTTCTACACAGACAATGG(+AAAAAA)GATCCAGAGGGTCTGCGGCAAGAAGGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606133256_plzy AGTTATTGGACCTTCTTGCCGCAGACCCTCTG(+AAAAAA)GATCCCATTGTCTGTGTAGAACGGGTCTATG NGG 10 8 17 8 24 0 100 5 10
    240606133255_lgx1 GTGACATCGAGATCAATTCAGGAGTCCAAGAG(+AAAAAA)GTACCTGTAAACTTAAACACAAGTCTGGACT NGG 10 8 17 8 24 0 100 5 10
    240606133254_zij2 GATGCAGTCCAGACTTGTGTTTAAGTTTACAG(+AAAAAA)GTACCTCTTGGACTCCTGAATTGATCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240606133252_r6hj GAACTAACTCACAGGCTCTTGTCAAAGTCTGG(+AAAAAA)GTACCAAATTTAGAAGTCTTTTTGATGTACC NGG 10 8 17 8 24 0 100 5 10
    240606133251_4g3c AAGAGGGTACATCAAAAAGACTTCTAAATTTG(+AAAAAA)GTACCCAGACTTTGACAAGAGCCTGTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606133250_0ijh TCTACTTAATTAGCGACCAGCAAACACTAATG(+AAAAAA)GATCCATGGGGTAGGGTTTTTGGTTTTGTTG NGG 10 8 17 8 24 0 100 5 10