Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133159_bdaf CTCTGGGATTCAGAGTAGAACGCTAAGATCAG(+AAAAAA)GATCCCGAGGAAGGCTCTGTGTCATGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240606133158_x9g7 TGTGCTCACTCCCTGGTGAACAGAGGAGCAGG(+AAAAAA)GATCCCAGAAAATAAAACGTGGAGGTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240606133156_d9ke TGGAAAAGAGACCTCCACGTTTTATTTTCTGG(+AAAAAA)GATCCCTGCTCCTCTGTTCACCAGGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240606133155_89qs CAGTGAGCAGCATATTTTATATACTGGTTGAG(+AAAAAA)GATCCCTAGTGAACATTATGACATCTGAACT NGG 10 8 17 8 24 0 100 5 10
    240606133153_svpd TTCATAGTTCAGATGTCATAATGTTCACTAGG(+AAAAAA)GATCCTCAACCAGTATATAAAATATGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240606133152_ntl2 GGGCTTGACTCTGATTGATGGCCTCTGGAGAG(+AAAAAA)GATCCTGCAGCTACCCAGATTCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606133151_c6z9 TTGGAGCCAACCTTGAATCTGGGTAGCTGCAG(+AAAAAA)GATCCTCTCCAGAGGCCATCAATCAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606133150_bdd5 ACAGGGTAATTCATTAGCAAGACATTTCATTG(+AAAAAA)GTACCGGGTTCAGGTTGTCTGGGGAATAATG NGG 10 8 17 8 24 0 100 5 10
    240606133147_3bs0 CCTCCCATTATTCCCCAGACAACCTGAACCCG(+AAAAAA)GTACCAATGAAATGTCTTGCTAATGAATTAC NGG 10 8 17 8 24 0 100 5 10
    240606133144_ydwc CCCCCGGGTCCAGCTCTGCTCCTGTGCCTGGG(+AAAAAA)GATCCGGAGGCTCCTGGCTGTCTTGAAATGG NGG 10 8 17 8 24 0 100 5 10