Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133109_npy6 AGGATTGCCAATAAGAAATAGGTAAATGTCAG(+AAAAAA)GATCCAGACTCCAGCAGATACACTGGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606133107_cmgz AGTCCCAACACCTATGCGTGTTCCCAACTCCG(+AAAAAA)GATCCTGTTAATACTATGGTTAAAAGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240606133106_dmmh TATGGAACCACTTTTAACCATAGTATTAACAG(+AAAAAA)GATCCGGAGTTGGGAACACGCATAGGTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606133104_p8eh TCTGGGCAGTAAGGACCCTATCACTGAGCCAG(+AAAAAA)GTACCTTGTCCTCTTCTTATTTTGAAGCATC NGG 10 8 17 8 24 0 100 5 10
    240606133103_y09i AAAATGATGCTTCAAAATAAGAAGAGGACAAG(+AAAAAA)GTACCTGGCTCAGTGATAGGGTCCTTACTGC NGG 10 8 17 8 24 0 100 5 10
    240606133102_plgm ACAGCCAGCACAGGAAGGAGCCAGTCATTTCG(+AAAAAA)GTACCTCTGAGGTTCTGAGAGTCGAGGGATA NGG 10 8 17 8 24 0 100 5 10
    240606133101_v2sz TGAGTTATCCCTCGACTCTCAGAACCTCAGAG(+AAAAAA)GTACCGAAATGACTGGCTCCTTCCTGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606133059_9q50 CTATACTCCCAGAACTTAGGATGCTGAAGGAG(+AAAAAA)GATCCAGAGTTGGAGGATAGCCTGGGCTACA NGG 10 8 17 8 24 0 100 5 10
    240606133058_40w3 CACTATGTAGCCCAGGCTATCCTCCAACTCTG(+AAAAAA)GATCCTCCTTCAGCATCCTAAGTTCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606133056_1g8t CTGGAGGGCTAGTCCCTAGACGCCGCCCTCTG(+AAAAAA)GATCCGTGCGTCTGAGGACAGCACCCTTGCT NGG 10 8 17 8 24 0 100 5 10