Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133055_7hwh GACAGAGCAAGGGTGCTGTCCTCAGACGCACG(+AAAAAA)GATCCAGAGGGCGGCGTCTAGGGACTAGCCC NGG 10 8 17 8 24 0 100 5 10
    240606133053_e9sz TTCCTTGGGTAACACCACACATACAGGTATGG(+AAAAAA)GATCCCACAACAGACAAATGTACAGACACCA NGG 10 8 17 8 24 0 100 5 10
    240606133051_tl5g TTTGGTGGTGTCTGTACATTTGTCTGTTGTGG(+AAAAAA)GATCCCATACCTGTATGTGTGGTGTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240606133050_mthn GGATGGCGGGCAAGGCCTATTAGTTCATCACG(+AAAAAA)GATCCGGTTCAGGTGCCCTAGAGCCCCGCTG NGG 10 8 17 8 24 0 100 5 10
    240606133048_ty0w CTAAGCAGCGGGGCTCTAGGGCACCTGAACCG(+AAAAAA)GATCCGTGATGAACTAATAGGCCTTGCCCGC NGG 10 8 17 8 24 0 100 5 10
    240606133046_a5tc CCCTGACAAAACTCATTCTACCCAGTACCCAG(+AAAAAA)GATCCTGGGCACACGCACATCTATATGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606133043_jy78 AACCATTTCCATATAGATGTGCGTGTGCCCAG(+AAAAAA)GATCCTGGGTACTGGGTAGAATGAGTTTTGT NGG 10 8 17 8 24 0 100 5 10
    240606133042_namv GAGGATTGACTCCTCAGAGAAGGAGCCTATGG(+AAAAAA)GTACCTGGGGCCACGTACAGCAGGGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240606133039_96po CTGCCGCTGAGCCCTGCTGTACGTGGCCCCAG(+AAAAAA)GTACCCATAGGCTCCTTCTCTGAGGAGTCAA NGG 10 8 17 8 24 0 100 5 10
    240606133038_x0p3 CTTGGAAAGATGGGCCACATGCTGCATGACAG(+AAAAAA)GATCCTCCAAGTACCTTAACTGTTAGAGTAT NGG 10 8 17 8 24 0 100 5 10