Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132912_udfc CAGCTAGTTTATGTGTCCATTTTCAATACCAG(+AAAAAA)GTACCCCCATGCCAATAGTACCATTCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606132911_3xuo GGGGATCTGATGTCCTCTTCTGGCCTCTGTTG(+AAAAAA)GTACCTGCACACACATGGTGTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240606132910_3w1q TATTTGTGTATGTGTACACCATGTGTGTGCAG(+AAAAAA)GTACCAACAGAGGCCAGAAGAGGACATCAGA NGG 10 8 17 8 24 0 100 5 10
    240606132908_rn2l GATTCTAGTCTGACTTGGAACAGAGGTCACAG(+AAAAAA)GTACCAACCTGATGAGACCTGCTGCTATTGT NGG 10 8 17 8 24 0 100 5 10
    240606132907_eiyf GAGACACAATAGCAGCAGGTCTCATCAGGTTG(+AAAAAA)GTACCTGTGACCTCTGTTCCAAGTCAGACTA NGG 10 8 17 8 24 0 100 5 10
    240606132906_4zz0 AATACATTATAGCATGTCCTGTAGGTCTGAAG(+AAAAAA)GTACCATAGTTCAGGTACTCACATGATTTAC NGG 10 8 17 8 24 0 100 5 10
    240606132904_ewbv GGTCTGTAAATCATGTGAGTACCTGAACTATG(+AAAAAA)GTACCTTCAGACCTACAGGACATGCTATAAT NGG 10 8 17 8 24 0 100 5 10
    240606132903_bpdr GGTGTCTGTGTTTGGTGGCTGATTATGGGATG(+AAAAAA)GATCCCTGGGTGGGGTAGTCTCTGGATGCAT NGG 10 8 17 8 24 0 100 5 10
    240606132900_ku1a GTAAAATGCATCCAGAGACTACCCCACCCAGG(+AAAAAA)GATCCATCCCATAATCAGCCACCAAACACAG NGG 10 8 17 8 24 0 100 5 10
    240606132859_p3dy TTGCACATGAGTAGAAGGACCTGAGTTTGATG(+AAAAAA)GATCCTGAGCCAGGGATGTGGCAGGAATTTA NGG 10 8 17 8 24 0 100 5 10