Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132857_x688 GATTATAAATTCCTGCCACATCCCTGGCTCAG(+AAAAAA)GATCCATCAAACTCAGGTCCTTCTACTCATG NGG 10 8 17 8 24 0 100 5 10
    240606132856_ymb9 TTTCGCGTGGGTAGAAGGACCTGAGTTTGATG(+AAAAAA)GATCCTGAGCCAGGGATGTGGCAGGAATTTA NGG 10 8 17 8 24 0 100 5 10
    240606132855_f4kg GATTATAAATTCCTGCCACATCCCTGGCTCAG(+AAAAAA)GATCCATCAAACTCAGGTCCTTCTACCCACG NGG 10 8 17 8 24 0 100 5 10
    240606132853_1qa4 CCTGACGCTTTCCACAATACACACGAGAAGGG(+AAAAAA)GTACCCCAGAGGCTAGGCTTTTCCAATGAAA NGG 10 8 17 8 24 0 100 5 10
    240606132851_9ofx AGACTTTTCATTGGAAAAGCCTAGCCTCTGGG(+AAAAAA)GTACCCCTTCTCGTGTGTATTGTGGAAAGCG NGG 10 8 17 8 24 0 100 5 10
    240606132849_dmyz GGAAGCCAGGCAGGACAGGTCCATGGGGCCTG(+AAAAAA)GTACCCACTGGCCAAAAATAACAGTGTGATT NGG 10 8 17 8 24 0 100 5 10
    240606132847_wfha AAAAAAATCACACTGTTATTTTTGGCCAGTGG(+AAAAAA)GTACCAGGCCCCATGGACCTGTCCTGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606132845_5htu CAAAGCAGCAGAGAATAGAGGACGGAGCCCAG(+AAAAAA)GTACCTCCTCTTCCTCCTCCTCACCGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240606132843_yr6i ATGGAGGCGACGGTGAGGAGGAGGAAGAGGAG(+AAAAAA)GTACCTGGGCTCCGTCCTCTATTCTCTGCTG NGG 10 8 17 8 24 0 100 5 10
    240606132841_h7sw TGGGAAATAGACCCTGCAGTTACCTAGAATGG(+AAAAAA)GTACCAGAGAGATGATGCTGGAAGCGCCGAC NGG 10 8 17 8 24 0 100 5 10