Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132755_p9wx TCCACAATAAAGAGACATAGACTAACAGACTG(+AAAAAA)GATCCATAAACAGTACCCAGCATTTTGCTGC NGG 10 8 17 8 24 0 100 5 10
    240606132753_w4rc AGACGAATGCCGAAGCCTGGCATCAGGGACGG(+AAAAAA)GATCCCATCCTCTGGAGTAGGCAATAGGTGG NGG 10 8 17 8 24 0 100 5 10
    240606132752_e4wq GTTTTCCACCTATTGCCTACTCCAGAGGATGG(+AAAAAA)GATCCCGTCCCTGATGCCAGGCTTCGGCATT NGG 10 8 17 8 24 0 100 5 10
    240606132750_c9t7 AGGGGTTCCAGATGCTCAGCAGACACTCCTGG(+AAAAAA)GATCCAAGGAAGCACTTATGCCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606132748_q3u4 CCCCATTCCTGGTTGGCATAAGTGCTTCCTTG(+AAAAAA)GATCCCAGGAGTGTCTGCTGAGCATCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606132747_29pp ACCCTGAGTAGCAGGTGGACTTGTTGGGATGG(+AAAAAA)GATCCAAGCCTGAGGGCTTACCCCATGTGGT NGG 10 8 17 8 24 0 100 5 10
    240606132745_bjdy CTGCCACCACATGGGGTAAGCCCTCAGGCTTG(+AAAAAA)GATCCCATCCCAACAAGTCCACCTGCTACTC NGG 10 8 17 8 24 0 100 5 10
    240606132744_s61g GTTAAGTTGTCTCTGTGATCCTGTGACTCTGG(+AAAAAA)GATCCTGGGACCCTGAGATCCTGGTGTGACC NGG 10 8 17 8 24 0 100 5 10
    240606132742_bdfw AGCTTGGTCACACCAGGATCTCAGGGTCCCAG(+AAAAAA)GATCCCAGAGTCACAGGATCACAGAGACAAC NGG 10 8 17 8 24 0 100 5 10
    240606132740_21te GACCGATGAGAGAGATCCGAGTGGGTTTCCTG(+AAAAAA)GATCCCACAAAAATTTCTTCATTCTTACAAA NGG 10 8 17 8 24 0 100 5 10