Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132720_rppw TGCTTGTGTGTGGAGGCCGTCGGCTGACCTGG(+AAAAAA)GTACCTCCTTCGTGCTCTCTGCCTTAATTTT NGG 10 8 17 8 24 0 100 5 10
    240606132719_scid TGCTCTGCCTCTCTGCTCAGGAACTAAGGATG(+AAAAAA)GTACCCTTTATCCAAAGCAGGAAGTTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240606132717_8xog GTTTCCTCACAACTTCCTGCTTTGGATAAAGG(+AAAAAA)GTACCATCCTTAGTTCCTGAGCAGAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606132715_yxa3 CAAAGGTCTAGAAAGGAAGGTTGCTTGGGCTG(+AAAAAA)GATCCCTTCCCCTCCTTTTCTCCGATTAAAT NGG 10 8 17 8 24 0 100 5 10
    240606132714_w8pk TTCGTATTTAATCGGAGAAAAGGAGGGGAAGG(+AAAAAA)GATCCAGCCCAAGCAACCTTCCTTTCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606132712_ima8 CGCTAACACATACCCTCAGCCCCTCACTGGGG(+AAAAAA)GATCCCAAAGTGCTCTACATCTCTAGATCTT NGG 10 8 17 8 24 0 100 5 10
    240606132710_6a3f TTTGAAAGATCTAGAGATGTAGAGCACTTTGG(+AAAAAA)GATCCCCCAGTGAGGGGCTGAGGGTATGTGT NGG 10 8 17 8 24 0 100 5 10
    240606132708_xmxy CATTTCTCTCCATTTACCACGTGGCTCCTGAG(+AAAAAA)GATCCAACTCAGGTTATCAGGTAGGTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606132707_mltj ACACTTAACCACCTACCTGATAACCTGAGTTG(+AAAAAA)GATCCTCAGGAGCCACGTGGTAAATGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240606132705_8x0f CACCAGGAGCCATTCCTGGCTGCATCCCATTG(+AAAAAA)GTACCCAAATACCGGAAGCCTTGACGATGGA NGG 10 8 17 8 24 0 100 5 10