Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132630_op0x TGGAAACCCCAATTGACAACAAAAATACCCAG(+AAAAAA)GTACCTGGGTAAATGTTTAACACAAGATGGA NGG 10 8 17 8 24 0 100 5 10
    240606132629_x8my AGCAAGGCCCTGTGTTCTTGACATCTGAGAGG(+AAAAAA)GATCCTACATATCAAGAGGGTCCGGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240606132627_rapj AGACAAGGAGCCCGGACCCTCTTGATATGTAG(+AAAAAA)GATCCCTCTCAGATGTCAAGAACACAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606132626_a1e9 AGGTGATCCAAATGCCCTTTTGGCCCCTGCGG(+AAAAAA)GTACCACATGTATGTGGTATGTGGATCTACA NGG 10 8 17 8 24 0 100 5 10
    240606132624_3iz2 CCACATGTAGATCCACATACCACATACATGTG(+AAAAAA)GTACCCGCAGGGGCCAAAAGGGCATTTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606132623_bjny TCTCAATTAGTACTTATAGGGATGGGATTGCG(+AAAAAA)GTACCACGGATACTGAAGCCCTCCCTGACTC NGG 10 8 17 8 24 0 100 5 10
    240606132621_a18e GTCTAGAGTCAGGGAGGGCTTCAGTATCCGTG(+AAAAAA)GTACCGCAATCCCATCCCTATAAGTACTAAT NGG 10 8 17 8 24 0 100 5 10
    240606132619_pen9 AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+AAAAAA)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10
    240606132618_nz7f GTGATATTTGTAAATAAAGGTAGAAACATTGG(+AAAAAA)GATCCACCCCTGCAGAACTTACTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240606132616_azes AACTAAACTGTACAGTAAGTTCTGCAGGGGTG(+AAAAAA)GATCCCAATGTTTCTACCTTTATTTACAAAT NGG 10 8 17 8 24 0 100 5 10