Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132544_r1z8 TCCCTCCCTTCCTGCTAAGCCACTGGTGTGGG(+AAAAAA)GATCCACCTCCGGCTGCTCCGGCACATGGCT NGG 10 8 17 8 24 0 100 5 10
    240606132543_9jff AAACCCAACAGTATCCTGAAGAAGACAGTGTG(+AAAAAA)GATCCATGTTTAAATAAGGAGATGGGACAGG NGG 10 8 17 8 24 0 100 5 10
    240606132541_qlwt ACCACCCTGTCCCATCTCCTTATTTAAACATG(+AAAAAA)GATCCACACTGTCTTCTTCAGGATACTGTTG NGG 10 8 17 8 24 0 100 5 10
    240606132540_yu50 TGCAAAGACAACAGTGTTTTAAAGTGGATTGG(+AAAAAA)GTACCAGGCTCACAAGCCTAGGTGATGTGCT NGG 10 8 17 8 24 0 100 5 10
    240606132538_q99o ACCACAGCACATCACCTAGGCTTGTGAGCCTG(+AAAAAA)GTACCCAATCCACTTTAAAACACTGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606132536_nap7 AAGCATCATCCTGGTTCTCTGCCGGCTGCCAG(+AAAAAA)GTACCCTAGTGCCAAGCACGTCTGGTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240606132534_mmhv AGCATCAGGCACCAGACGTGCTTGGCACTAGG(+AAAAAA)GTACCTGGCAGCCGGCAGAGAACCAGGATGA NGG 10 8 17 8 24 0 100 5 10
    240606132533_w9k8 CATTTTTATCTCACAGTTACACCCTCCTGTCG(+AAAAAA)GATCCTCCTCCAAGTCTGACCACTGACTGGG NGG 10 8 17 8 24 0 100 5 10
    240606132531_zv8c GCTAGCCCAGTCAGTGGTCAGACTTGGAGGAG(+AAAAAA)GATCCGACAGGAGGGTGTAACTGTGAGATAA NGG 10 8 17 8 24 0 100 5 10
    240606132530_35yp TAGCTGTATCTTTCTCACTCAGGTAGAAATGG(+AAAAAA)GATCCACCCTGCTTGACCTTTTCCAGAAGGT NGG 10 8 17 8 24 0 100 5 10