Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132455_sajy GCAAAAAATAAACCTAGTCTCTGGAGGAGCCG(+AAAAAA)GTACCCTCCGAGGGAGCCAAGGGCGTGCCCA NGG 10 8 17 8 24 0 100 5 10
    240606132453_xnse CTAAAGGGTGGTGACTGGCTTCAGGGGGTGTG(+AAAAAA)GTACCATCTGTGCCTGCTGCCAAGACGCAGG NGG 10 8 17 8 24 0 100 5 10
    240606132452_8e66 TTGATCCTGCGTCTTGGCAGCAGGCACAGATG(+AAAAAA)GTACCACACCCCCTGAAGCCAGTCACCACCC NGG 10 8 17 8 24 0 100 5 10
    240606132451_q8hl GGAAGTATAGCCAAGATAGGCCCTGGGTCATG(+AAAAAA)GTACCTGCTCACTGGAGGTCTGCAAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240606132449_fhid CTGGGCCTACTTTGCAGACCTCCAGTGAGCAG(+AAAAAA)GTACCATGACCCAGGGCCTATCTTGGCTATA NGG 10 8 17 8 24 0 100 5 10
    240606132448_dbl6 TTACACTGCTTCACAAAAGGACGGGGCATGAG(+AAAAAA)GATCCCCTTGTCTATTTATAAAAGCAATAAA NGG 10 8 17 8 24 0 100 5 10
    240606132446_gfyu GTCATTTTATTGCTTTTATAAATAGACAAGGG(+AAAAAA)GATCCTCATGCCCCGTCCTTTTGTGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606132445_js5q TGGGAATCATTGTCCAGGAGTAACTCCAGCAG(+AAAAAA)GATCCCAGGTCTCTGCAGAAGCAGGAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240606132442_8cxd CAAGCCACCTTCCTGCTTCTGCAGAGACCTGG(+AAAAAA)GATCCTGCTGGAGTTACTCCTGGACAATGAT NGG 10 8 17 8 24 0 100 5 10
    240606132441_11lm CTCTTCCCAAAGAGCCACTTACCTCTGGACAG(+AAAAAA)GTACCCTGCGTCTGGTTCACGGTGACGATCA NGG 10 8 17 8 24 0 100 5 10