Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132352_l8vo TGTTTCAAAGTTACATGTGTCAAAAATTCCAG(+AAAAAA)GTACCTTTTATTTTCATCATACCTATGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606132350_ayx5 ACTGCTAGGCTTTTTGATGCAAGTTGGATTGG(+AAAAAA)GATCCTTCCAGGTCTCTTCTTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240606132349_po99 CTTGTGTGTATGTGTAAGAAGAGACCTGGAAG(+AAAAAA)GATCCCAATCCAACTTGCATCAAAAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240606132347_32v7 CTGTGGAAGAGTTTCTATGTGAGATGAGGATG(+AAAAAA)GATCCCTCAGAGTACACACCCATCTCATGGC NGG 10 8 17 8 24 0 100 5 10
    240606132346_gzdc AGCAAGCCATGAGATGGGTGTGTACTCTGAGG(+AAAAAA)GATCCATCCTCATCTCACATAGAAACTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606132344_y1fs ACTGTTACACGTATTGATGTTGGGTAGCTTGG(+AAAAAA)GATCCTTCTAGATCTCTGATGCAAGAAACCC NGG 10 8 17 8 24 0 100 5 10
    240606132343_2sh2 AGTCTGGGTTTCTTGCATCAGAGATCTAGAAG(+AAAAAA)GATCCCAAGCTACCCAACATCAATACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606132342_mva0 GTACAGTGGATCTGAGCGGCAGGGGATTATTG(+AAAAAA)GTACCATCTGCTCTTCGGGCCCAACGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240606132341_asne GGGACTACCCCGTTGGGCCCGAAGAGCAGATG(+AAAAAA)GTACCAATAATCCCCTGCCGCTCAGATCCAC NGG 10 8 17 8 24 0 100 5 10
    240606132338_ubpa TGCCTGGTTCTAGGCCCCTTCCTCTACCCCAG(+AAAAAA)GTACCCTCAGCCTAGGGGAGCAGGCTCAGGT NGG 10 8 17 8 24 0 100 5 10