Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132315_520o ACCAGAGGCTTCAAAGGCGAGTCTTTGGCTGG(+AAAAAA)GTACCCTAGCACAGCCTCTCTCCATGGACTC NGG 10 8 17 8 24 0 100 5 10
    240606132313_48jn GGCGTGGCCCCATCTTTGGGAACCTGGTGTGG(+AAAAAA)GATCCAGGCAAATCTGGAAGGCAAAAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606132312_1b1q CCTATGAACTTTTTGCCTTCCAGATTTGCCTG(+AAAAAA)GATCCCACACCAGGTTCCCAAAGATGGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606132309_jmgx AGACTGCACCTTTGTGATTCCTTTTCTCCCGG(+AAAAAA)GATCCACCTGGGACAGTCAGTCTCAGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240606132307_z10y GCTGACCCTTCTGAGACTGACTGTCCCAGGTG(+AAAAAA)GATCCCGGGAGAAAAGGAATCACAAAGGTGC NGG 10 8 17 8 24 0 100 5 10
    240606132306_leee CCAGAGCCACTTGATCTGATACAACAGAGCGG(+AAAAAA)GTACCACGTTGGCTTGGGGGTGGGTGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606132305_x7n2 GCCGCTCCCCCACCCACCCCCAAGCCAACGTG(+AAAAAA)GTACCCGCTCTGTTGTATCAGATCAAGTGGC NGG 10 8 17 8 24 0 100 5 10
    240606132302_axxh CTGATGCTTTCTAGCAGACCCTCTGATCCCAG(+AAAAAA)GTACCTGGCAAGTCATCTGCAAATCCAACCA NGG 10 8 17 8 24 0 100 5 10
    240606132300_7frv TTTCCTGGTTGGATTTGCAGATGACTTGCCAG(+AAAAAA)GTACCTGGGATCAGAGGGTCTGCTAGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240606132258_kw05 AGCAATGGCTCTTACCTGCCCACAAAGGACAG(+AAAAAA)GTACCCAACCCCCGTGCTAGGACTGGAACCC NGG 10 8 17 8 24 0 100 5 10