Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132203_mzar CACCAACATCCACCTGCCCCTGCTCCTGTCTG(+AAAAAA)GATCCAAGCTCTGAGCTCTTATCTTGAATTT NGG 10 8 17 8 24 0 100 5 10
    240606132201_268d GACATTGTGCTCATCTCGATGTCAGCAAAAGG(+AAAAAA)GATCCACTCCTTCCTATGAGCAAACCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606132200_1qfl AAACAGACCAGGTTTGCTCATAGGAAGGAGTG(+AAAAAA)GATCCCTTTTGCTGACATCGAGATGAGCACA NGG 10 8 17 8 24 0 100 5 10
    240606132158_havz CTTTGCTGCTGCAAGGAGAATGTCTCTCTTGG(+AAAAAA)GTACCAGTGGGTCCTTCACACCTTCCCACAA NGG 10 8 17 8 24 0 100 5 10
    240606132157_32gq TGGTGTTGTGGGAAGGTGTGAAGGACCCACTG(+AAAAAA)GTACCCAAGAGAGACATTCTCCTTGCAGCAG NGG 10 8 17 8 24 0 100 5 10
    240606132155_ucte GCACACACTCCCAAAGAAGTTTCTGAGGAATG(+AAAAAA)GATCCTTGATTTTAAAACTGTAAGATCTTTT NGG 10 8 17 8 24 0 100 5 10
    240606132154_yjvs GGAGGAAAAGATCTTACAGTTTTAAAATCAAG(+AAAAAA)GATCCATTCCTCAGAAACTTCTTTGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240606132153_5tl4 TACAGGGATGCCACCACCATTCCAGCATATTG(+AAAAAA)GATCCTGATTCTTATATCCTGTTTGTGTGTT NGG 10 8 17 8 24 0 100 5 10
    240606132151_9n45 ACTGAAACACACAAACAGGATATAAGAATCAG(+AAAAAA)GATCCAATATGCTGGAATGGTGGTGGCATCC NGG 10 8 17 8 24 0 100 5 10
    240606132150_i588 AGGCACAGTATTTGGTTAAGCCTGAGTTTTGG(+AAAAAA)GTACCTTTTGTTACTGTTTTCAAAACCCTGA NGG 10 8 17 8 24 0 100 5 10