Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132148_jwcp GCAAGTCAGGGTTTTGAAAACAGTAACAAAAG(+AAAAAA)GTACCCAAAACTCAGGCTTAACCAAATACTG NGG 10 8 17 8 24 0 100 5 10
    240606132146_5dhq CCCAAGCCAAGCCTCAGCCTGGGCTTTCCCAG(+AAAAAA)GATCCTGTTAGTGAGCAGGGCTCAGCCACTC NGG 10 8 17 8 24 0 100 5 10
    240606132144_dk2w GGTCTGAGTGGCTGAGCCCTGCTCACTAACAG(+AAAAAA)GATCCTGGGAAAGCCCAGGCTGAGGCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606132142_a36s TGCAGCCACTGCCAGAGCTGACCTTGACTGGG(+AAAAAA)GATCCTTGTCTGGGACCCCATCACGCTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606132141_zznb TAGAGGACCAGCGTGATGGGGTCCCAGACAAG(+AAAAAA)GATCCCCAGTCAAGGTCAGCTCTGGCAGTGG NGG 10 8 17 8 24 0 100 5 10
    240606132139_x4k2 TAACTTGTTTCATTTCTTTTCAGATCCCTAAG(+AAAAAA)GATCCCCCCACCCCCCAATGCCGCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240606132136_3v9j TTCAGAGTTGGAGCGGCATTGGGGGGTGGGGG(+AAAAAA)GATCCTTAGGGATCTGAAAAGAAATGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606132135_txjm TTCAGAGCCAGCACATCACCATCTGTTTCTGG(+AAAAAA)GTACCTACCTTATGCCTACCAGAGGGCTCGT NGG 10 8 17 8 24 0 100 5 10
    240606132133_w7m1 ATAAAACGAGCCCTCTGGTAGGCATAAGGTAG(+AAAAAA)GTACCCAGAAACAGATGGTGATGTGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606132132_wugs GGTGTCTGACCCTCCACCACCTCACCTTGTTG(+AAAAAA)GTACCTCCCTCCCTGAATTCCTGAGCCAGCA NGG 10 8 17 8 24 0 100 5 10