Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132238_fhy7 GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+AAAAAA)GATCCTATTACAGATGGTTGTGAGCCACTCG NGG 10 8 17 8 24 0 100 5 10
    240606132235_gaqi ATACCAGGACCCACGTGTGCTAGTGTCCCTGG(+AAAAAA)GATCCCAGTTCACAGAAGAGCACCAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240606132233_wnhd CTTCCAGGCCTTGGTGCTCTTCTGTGAACTGG(+AAAAAA)GATCCCAGGGACACTAGCACACGTGGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240606132231_gpo2 CCTTAAGCTTATCTGCCTATTGATATCCCAGG(+AAAAAA)GATCCTGAAACAGGCCCCTGCGCATACAGAG NGG 10 8 17 8 24 0 100 5 10
    240606132229_i90z TGTTCCTCTGTATGCGCAGGGGCCTGTTTCAG(+AAAAAA)GATCCCTGGGATATCAATAGGCAGATAAGCT NGG 10 8 17 8 24 0 100 5 10
    240606132228_c2hv GGATTGCCAGCCTCCCTCAGCAGGGGCACTTG(+AAAAAA)GTACCCCCATAAGGGTGGCATGCCCCAGTCC NGG 10 8 17 8 24 0 100 5 10
    240606132225_1iro CAAGTGGACTGGGGCATGCCACCCTTATGGGG(+AAAAAA)GTACCAAGTGCCCCTGCTGAGGGAGGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240606132224_g99x AAAAGAGGGGGGTGGGGGAGCTCCATGGGGTG(+AAAAAA)GATCCCAGGCTGGCAGCAGGAAGGTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240606132221_lo4i GGCCTTCCAGCACCTTCCTGCTGCCAGCCTGG(+AAAAAA)GATCCACCCCATGGAGCTCCCCCACCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240606132220_ezaf TAAAAAGAAACTTATGATTTATTAGAGTACAG(+AAAAAA)GATCCAAAACACATTTTTAGAAAAATATAAA NGG 10 8 17 8 24 0 100 5 10