Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606132336_keqk CTTCCACCTGAGCCTGCTCCCCTAGGCTGAGG(+AAAAAA)GTACCTGGGGTAGAGGAAGGGGCCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240606132334_ejcp CCCCCGGGAACCCCCACCCCCACCCCCGGCAG(+AAAAAA)GATCCCTCCATGCAGGAGAAAGCCAACCAGA NGG 10 8 17 8 24 0 100 5 10
    240606132333_0eti GGGCCTCTGGTTGGCTTTCTCCTGCATGGAGG(+AAAAAA)GATCCTGCCGGGGGTGGGGGTGGGGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240606132329_rgc4 TGTCGCCCAGGGCTGTAAGCTGAGACCCCTGG(+AAAAAA)GATCCCTGTCTAAGCTAGATGCCCCTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240606132328_9rs1 AATGGAGGGAAGGGGCATCTAGCTTAGACAGG(+AAAAAA)GATCCCAGGGGTCTCAGCTTACAGCCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240606132326_76sf CTGGGGCTTATGCTTGGCTGTGGGCCTGGTGG(+AAAAAA)GATCCGGGGCACACATTCTCCTAGTGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240606132324_5bjk GAGGGACCAGCACTAGGAGAATGTGTGCCCCG(+AAAAAA)GATCCCACCAGGCCCACAGCCAAGCATAAGC NGG 10 8 17 8 24 0 100 5 10
    240606132321_3251 CTCTTAGTTCAAGGAGGTGATGATGGGTGGGG(+AAAAAA)GATCCGGAGGGCTTCTAAGATGTGCTTGGAA NGG 10 8 17 8 24 0 100 5 10
    240606132319_2aql CGAGATTCCAAGCACATCTTAGAAGCCCTCCG(+AAAAAA)GATCCCCCACCCATCATCACCTCCTTGAACT NGG 10 8 17 8 24 0 100 5 10
    240606132317_fwac TGTCCGAGTCCATGGAGAGAGGCTGTGCTAGG(+AAAAAA)GTACCCAGCCAAAGACTCGCCTTTGAAGCCT NGG 10 8 17 8 24 0 100 5 10