Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606133407_d25h CGTCATCCTAAACTCACCATCCTTAGAAACGG(+AAAAAA)GATCCTCTCTAGAATATTGTCGCCGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240606133406_82p3 CCTGTGGTCACCGGCGACAATATTCTAGAGAG(+AAAAAA)GATCCCGTTTCTAAGGATGGTGAGTTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240606133405_1sr8 TTACTTTGATGGCTTCTCCAGGTTTTTGTCAG(+AAAAAA)GTACCACATGTAGAATTACATTTTTCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240606133404_dpts AATCTTTTAAGAAAAATGTAATTCTACATGTG(+AAAAAA)GTACCTGACAAAAACCTGGAGAAGCCATCAA NGG 10 8 17 8 24 0 100 5 10
    240606133402_k37y CCTCCATCCGGAGGAAGCAACAGCTGAATCAG(+AAAAAA)GATCCGCAGCGTAAGGGGTCAGCTAATCAGT NGG 10 8 17 8 24 0 100 5 10
    240606133401_c4qp CTCCCACTGATTAGCTGACCCCTTACGCTGCG(+AAAAAA)GATCCTGATTCAGCTGTTGCTTCCTCCGGAT NGG 10 8 17 8 24 0 100 5 10
    240606133400_8g5i AGCTAGACATTTATTGATAATACATTACAGTG(+AAAAAA)GATCCAAAATCTAGCATTATTTTAGTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240606133359_x4c9 CCTGTTCACCACTAAAATAATGCTAGATTTTG(+AAAAAA)GATCCACTGTAATGTATTATCAATAAATGTC NGG 10 8 17 8 24 0 100 5 10
    240606133357_yq45 ATGTGCCCATCAGGGGGCCGTCTTAACCTTTG(+AAAAAA)GTACCATCTTCTTCAAAGCTGCATTAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606133355_00hv TGCTCTCTTTTAATGCAGCTTTGAAGAAGATG(+AAAAAA)GTACCAAAGGTTAAGACGGCCCCCTGATGGG NGG 10 8 17 8 24 0 100 5 10